Journal: Theranostics
Article Title: Bioenergetic Crosstalk between Mesenchymal Stem Cells and various Ocular Cells through the intercellular trafficking of Mitochondria
doi: 10.7150/thno.46332
Figure Lengend Snippet: Mitochondria transfer from MSCs to corneal endothelial cells. A. Coculture of Mito-COX8-GFP-labeled CECs and violet-labeled CECs; (A') magnified image of box-A'. Arrowheads represent CEC and MSC. B. Coculture of GFP-MSCs and violet-CECs; (B') magnified image of box-B'. MSC-derived mitochondria (arrow) in recipient CECs (blue arrowheads). C-D. Representative images and intercellular mitochondrial transfer rate from GFP-CECs to violet-CECs that were pretreated with 0, 1 µm, and 5 µm rot. One-way ANOVA, mean ± SD, ns: no significant difference, n = 5. E-F. Representative images and rate of mitochondrial transfer from GFP-MSCs to violet-CECs that were pretreated with 0, 1 µm and 5 µm rot. One-way ANOVA, mean ± SD, * P < 0.05, n = 5. rot: rotenone.
Article Snippet: Human corneal endothelial cells (DSMZ, Cat# ACC-646, RRID: CVCL_2064) were cultured as previously reported .
Techniques: Labeling, Derivative Assay